Protein Purification Reagents and Kits
STEMCELL Technologies EasySep™ Human Biotin Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human Biotin Positive Selection Kit II is designed to isolate cells that are labeled with biotinylated antibodies. Desired cells are targeted with antibody complexes recognizing biotin and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Human Biotin Positive Selection Kit (Catalog #18553). For highly purified particle-free cells, we recommend the EasySep™ Release Human Biotin Positive Selection Kit (Catalog #17653). (https://www.stemcell.com/products/easysep-release-human-biotin-positive-selection-kit.html)
STEMCELL Technologies Mitochondrial Isolation Kit, Size: 50 Tests
Conveniently enrich the mitochondrial or cytosolic subcellular fractions from mammalian cells or tissues using the Mitochondrial Isolation Kit. This kit offers two quick options to isolate the mitochondrial subcellular fraction and has been verified for use with western blot analysis. The first option is a reagent-based method for processing up to six samples concurrently. The second option utilizes Dounce homogenization, providing a two-fold increase in isolated mitochondria from a single sample. Both options use a microcentrifuge-based method to separate the mitochondrial fraction from the cytosolic fraction while preserving their biological activity. This separation is a valuable tool for studying apoptosis and signaling events between the two fractions. For more information on the Mitochondrial Isolation Kit, visit www.stemcell.com.
Biolegend KIRAVIA Blue 520TM anti-mous
KIRAVIA Blue 520™ anti-mouse CD366 (Tim-3) [RMT3-23]; Isotype: Rat IgG2a, κ; Reactivity: Mouse; Apps: FC; Size: 25 ug
IW Tremont Co Inc LabExact CFP41 cellulose filter paper, 25um 15cm very fast flow rates ideal for initial separation of gelatinous precipitates, extremely low ash content of <0.01%. Thickness 0.220mm 100/pk
LabExact Very fast flow rates and loose retention characteristics make Grade CFP41 ideal for initial separation of gelatinous precipitates. Retention of approximately 20 um. Extremely low ash content of <0.01%. Thickness 0.220mm.
STEMCELL Technologies EasySep™ Human Resting CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Resting CD4+ T Cell Isolation Kit is designed to isolate resting CD4+ T cells from fresh or previously frozen human peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
Hampton Research HR8-130/CrystalCap HT, 0.5 - 0.7 mm - 30 pack
HR8-130/CrystalCap HT, 0.5 - 0.7 mm - 30 pack. Please note items from this supplier are non-returnable.
Medchemexpress LLC BID Mouse His-GST 1mg
BID Protein a pro-apoptotic member of the Bcl-2 family is initially discovered through binding to both pro-apoptotic Bax and anti-apoptotic Bcl-2 BID is activated in the BCL-2-regulated or mitochondrial apoptosis pathway and acts as a switch between the extrinsic and intrinsic cell death pathways BID is susceptible to proteolytic cleavage by caspases calpains Granzyme B and cathepsins BID Protein Mouse (His-GST) is the recombinant mouse-derived BID protein expressed by E coli with N-His N-GST labeled tag
Cayman Chemical Monoacylglycerol Lipase Inhib
Selectively and reversibly blocks MAGL activity (IC50s = 0.18 and 59 µM for MAGL and FAAH in mouse brain, respectively); does not inhibit ABHD6, ABHD12, CB1, or CB2 receptors (Kis > 10 µM); used to ameliorate disease progression in a mouse model of multiple sclerosis
Hampton Research HR2-406-52/Detergent Screen #52 (E4), 0.5 ml - 104.0 mM DDAO
HR2-406-52/Detergent Screen #52 (E4), 0.5 ml - 104.0 mM DDAO. Please note items from this supplier are non-returnable.
Hampton Research HR2-406-50/Detergent Screen #50 (E2), 0.5 ml - n-Octyl-B-D-thioglucoside
HR2-406-50/Detergent Screen #50 (E2), 0.5 ml - n-Octyl-B-D-thioglucoside. Please note items from this supplier are non-returnable.
Hampton Research HR2-406-48/Detergent Screen #48 (D12), 0.5 ml - 80.0 mM C8E4
HR2-406-48/Detergent Screen #48 (D12), 0.5 ml - 80.0 mM C8E4. Please note items from this supplier are non-returnable.
Hampton Research 10% W/V CYMAL-1 0.5ML
HR2-406-72/Detergent Screen #72 (F12), 0.5 ml - 10% w/v CYMAL-1. Please note items from this supplier are non-returnable.
Hampton Research 10% W/V ZWITTERGENT 3-0 0.5ML
HR2-406-94/Detergent Screen #94 (H10), 0.5 ml - 10% w/v ZWITTERGENT 3-08. Please note items from this supplier are non-returnable.
Enzo Life Sciences SUMO-QAPTURE-T kit (10 tests)
The SUMO-QAPTURE-T kit is an efficient tool for the selective isolation of SUMOylated proteins. The kit facilitates the affinity purification of SUMOylated proteins from cell extracts and tissue lysates using a high-binding SIM-containing affinity matrix. Captured proteins are eluted under denaturing conditions followed by analysis by Western blotting, using the SUMO antibodies provided or antibodies to specific proteins of interest, or potential substrate identification by proteomic methods. SUMO-conjugate-containing samples are prepared in native form in the presence of protease inhibitors to prevent loss through the action of deSUMOylating enzymes. Optimization of binding permits complete isolation of the full range of SUMO-protein conjugates from a specific lysate.